Anti-mouse IgA mAb (MT45A), unconjugated

Anti‑mouse IgA mAb (MT45A), unconjugated

3865-3-250

Publications: 28

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Content

Monoclonal antibody MT45A, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse IgA mAb (MT39A), ALP

Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgA in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

In ELISA, MT45A is recommended as capture mAb in combination with ALP-conjugated detection mAb MT39A (product code 3865-9A). In ELISpot, MT45A is recommended as capture mAb in combination with biotinylated detection mAb MT39A (product code 3865-6). The antibody is also recommended as capture mAb in antigen-specific ELISpot and ELISA (detection with biotinylated antigen).

Product details

Product	Anti-mouse IgA mAb (MT45A), unconjugated
Application	ELISpot, ELISA
Analyte	IgA
Antibody	MT45A
Conjugate	Unconjugated
Clonality	Monoclonal
Immunogen	Native mouse Ig
Host	Rat
Isotype	IgG2a
Reactivity	Mouse
Specificity	Mouse IgA
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Concentration	0.5 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody MT45A, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Publications (28)

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Analyte information

IgA

Analyte description	IgA (Immunoglobulin A) represents only 10-15% of the total Ig molecules in serum but is the predominating Ig isotype in secretions and in the gastrointestinal and urogenital tract. The IgA found in secretions (secretory IgA or sIgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.
Alternative names	Immunoglobulin A, IgA, Igh-2
Cell type	B cell
Gene ID	238447