ELISpot Flex: Mouse IgA (ALP)

ELISpot Flex: Mouse IgA (ALP)

3865-2A

Publications: 25
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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT45A
Detection mAbMT39A, biotin
Enzyme conjugateStreptavidin-ALP
StimuliR848
Recombinant mouse IL-2

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Delivery 4-9 business days

Shipping $0

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Complementary products
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ELISpot plates, white


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of B cells secreting mouse IgA using the ELISpot assay. B cells secreting antigen-specific IgA, as well as B cells secreting IgA irrespective of antigen specificity (total IgA) can be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step. For memory B cells, we recommend pre-stimulation with R848 and recombinant mouse IL-2 (both included).

Recommendation

Product details

ProductELISpot Flex: Mouse IgA (ALP)
ApplicationELISpot
AnalyteIgA
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of mouse IgA.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. R848 and IL-2 should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells were pre-incubated for 72 h in the absence or presence of R848 and recombinant IL-2. The cells were then washed, added into ELISpot wells and analyzed for the secretion of total IgA (80,000 cells/well, overnight incubation).
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot
ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published October 14, 2022

Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IgA

Analyte description

IgA (Immunoglobulin A) represents only 10-15% of the total Ig molecules in serum but is the predominating Ig isotype in secretions and in the gastrointestinal and urogenital tract. The IgA found in secretions (secretory IgA or sIgA) is commonly a dimer with two IgA molecules joined by the J chain. Secretory IgA is the most important Ig isotype in mucous surfaces, the area of entry for most pathogens.

Alternative namesImmunoglobulin A, IgA, Igh-2
Cell typeB cell
Gene ID238447