Anti-mouse IgE mAb (MT56E), unconjugated

Anti‑mouse IgE mAb (MT56E), unconjugated

3815-3-250

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Content

Monoclonal antibody MT56E, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgE in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT56E is recommended as capture mAb in ELISpot and ELISA in combination with biotinylated detection mAb MT44E (product code 3815-6).

Product details

ProductAnti-mouse IgE mAb (MT56E), unconjugated
ApplicationELISpot, ELISA
AnalyteIgE
AntibodyMT56E
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative mouse IgE
HostRat
IsotypeIgG1
ReactivityMouse
SpecificityMouse IgE. The antibody is specific for mouse IgE allotypes IgEa (allotype a) and IgEb (allotype b).
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT56E, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgE

Analyte description

The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.

Alternative namesImmunoglobulin E, IgE, Gm900
Cell typeB cell
Gene ID380792
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ELISA Flex: Mouse IgE (HRP)