Anti-mouse IgEa mAb (MT18E), unconjugated

Anti‑mouse IgEa mAb (MT18E), unconjugated

3816-3-250

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Content

Monoclonal antibody MT18E, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

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Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IgEa in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

MT18E is recommended as capture mAb in ELISpot and ELISA in combination with biotinylated detection mAb MT44E (product code 3815-6).

Product details

ProductAnti-mouse IgEa mAb (MT18E), unconjugated
ApplicationELISpot, ELISA
AnalyteIgEa
AntibodyMT18E
ConjugateUnconjugated
ClonalityMonoclonal
ImmunogenNative mouse IgE
HostRat
IsotypeIgG2a
ReactivityMouse
SpecificityMouse IgEa (allotype a). The antibody is specific for mouse IgEa (allotype a) and does not bind IgEb (allotype b).
PurificationPurified from in vitro cultures by protein G affinity chromatography.
Concentration0.5 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody MT18E, unconjugated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IgEa

Analyte description

Immunoglobulin E (IgE) E is secreted by plasma cells as a monomer during the adaptive immune response. IgE antibodies are believed to play an important role in the immune defense against parasitic infections and in hypersensitivity type I reactions upon re-exposure to a specific allergen by binding to and cross-linking Fc receptors (FcεRI) expressed on mast cells and basophils. Allergen-specific IgE is the basis for many in vitro assays for allergy diagnosis. In mice, two allotypes exist: IgEa and IgEb. IgEa differs by 12 single-base changes from IgEb and is present in different mouse strains.

Alternative namesImmunoglobulin Ea, IgEa, Gm900
Cell typeB cell
Gene ID380792
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