Anti-mouse IL-12 (p35) mAb (IL12-IV), biotin

Anti‑mouse IL‑12 (p35) mAb (IL12‑IV), biotin

3456-6-250

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Content

Monoclonal antibody IL12-IV, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse IL-12/-23 (p40) mAb (C17.8), unconjugated


Product specifications

Intended use

This monoclonal antibody enables specific detection of mouse IL-12 (p70) in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

IL12-IV is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb C17.8 (product code 3456-3).

Product details

ProductAnti-mouse IL-12 (p35) mAb (IL12-IV), biotin
ApplicationELISpot, ELISA
AnalyteIL-12 (p70)
AntibodyIL12-IV
ConjugateBiotin
ClonalityMonoclonal
ImmunogenRecombinant mouse IL-12
HostRat
IsotypeIgG2a
ReactivityMouse
SpecificityNative and recombinant mouse IL-12 p70. The antibody recognizes the p35 subunit of IL-12 p70 heterodimer (p40 + p35).
PurificationPurified from in vitro cultures by protein G affinity chromatography.
BiotinylationBiotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration1 mg/ml
Supplied inPBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
ContentsMonoclonal antibody IL12-IV, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.
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IL-12 (p70)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Alternative namesInterleukin 12, IL-12, IL12, IL-12p35, Il-12a, Ll12a, p35, Il-12b, Il-12p40, Il12p40, p40
Cell typeMonocyte/MΦ, mDC
Gene ID16159, 16160
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ELISpot Plus: Mouse IL-12 p70 (ALP)