Anti-mouse IL-12/-23 (p40) mAb (C17.8), biotin

3451-6-250

Publications: 5

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Monoclonal antibody C17.8, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

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Anti-mouse IL-12/-23 (p40) mAb (C15.6), unconjugated

Product specifications

Intended use

This monoclonal antibody enables specific detection of the p40 subunit of mouse IL-12 and IL-23 in immunoassays such as ELISpot and ELISA.

Serum/Plasma samples

Recommendation

C17.8 is recommended as detection mAb in ELISpot and ELISA in combination with capture mAb C15.6 (product code 3451-3).

Product details

Product	Anti-mouse IL-12/-23 (p40) mAb (C17.8), biotin
Application	ELISpot, ELISA
Analyte	IL-12/-23 (p40)
Antibody	C17.8
Conjugate	Biotin
Clonality	Monoclonal
Immunogen	Recombinant mouse IL-12 p70
Host	Rat
Isotype	IgG2a
Reactivity	Mouse
Specificity	Native and recombinant p40 subunit of mouse IL-12 and IL-23. The antibody recognizes p40 monomer, p40 homodimer, IL-12 p70 heterodimer (p40+p35) and IL-23 heterodimer (p40+p19).
Purification	Purified from in vitro cultures by protein G affinity chromatography.
Biotinylation	Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Concentration	1 mg/ml
Supplied in	PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody C17.8, biotinylated. Supplied at 1 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
Shelf life	At least 18 months from date of receipt.

Documents

Tutorials

Tutorial, Published December 28, 2023

Publications (5)

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Analyte information

IL-12/-23 (p40)

Analyte description	Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells. Interleukin-23 (IL-23) is a disulfide-linked heterodimer that consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells, and IL-23 is responsible for Th17 expansion and stabilization. Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.
Alternative names	Interleukin 12, IL-12, IL12, IL12A, IL12B, Il-12b, Il-12p40, Il12p40, p40
Cell type	Monocyte/MΦ, mDC
Gene ID	16160