ELISA Flex: Cat IFN-γ (ALP)

ELISA Flex: Cat IFN‑γ (ALP)

3122-1A-6

Publications: 3
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT131
Detection mAbMT114, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant cat IFN-γ ELISA standard
Buffer/SolutionStandard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

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ELISA substrate: pNPP for ALP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of cat IFN-γ in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Cat IFN-γ (ALP)
ApplicationELISA
AnalyteIFN-γ
ReactivityCat, Lion, Cheetah, Leopard
Specificity

This kit is based on a matched pair of mAbs specific for cat IFN-γ.

Cross-reactivity

Cross-reacts with IFN-γ from lion, cheetah, and leopard.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range4-400 pg/ml
Representative standard curve for cat IFN-γ ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

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Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell typeT cell, Tc, Th1, NK cell
Gene ID493965