ELISA Flex: Bovine TNF-α (ALP)

ELISA Flex: Bovine TNF‑α (ALP)

3012-1A-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMTF4918
Detection mAbMT771, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant bovine TNF-α ELISA standard
Buffer/SolutionStandard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

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ELISA substrate: pNPP for ALP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of bovine TNF-α in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Bovine TNF-α (ALP)
ApplicationELISA
AnalyteTNF-α
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) TNF-α.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range10-3160 pg/ml

Representative standard curve for bovine TNF-ɑ ELISA Flex kit using pNPP substrate.

Datasheet/Protocol
SDS

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Featured product, Published September 14, 2023

Detect TNF-α secretion from cow cells
You can now analyze the secretion of bovine TNF-α with ELISA, ELISpot, and FluoroSpot.

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, TNF-a-alpha
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID280943