ELISA Flex: Human CCL2 (ALP)

ELISA Flex: Human CCL2 (ALP)

3491-1A-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMTBH5
Detection mAbMTBH9, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human CCL2 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human CCL2 (MCP-1) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

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Product details

ProductELISA Flex: Human CCL2 (ALP)
ApplicationELISA
AnalyteCCL2 (MCP-1)
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human CCL2 (MCP-1).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range2-500 pg/ml

Representative standard curve for CCL2 ELISA Flex kit using pNPP substrate.

Datasheet/Protocol
SDS

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Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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CCL2 (MCP-1)

Analyte description

Chemokine ligand 2 (CCL2), also called monocyte chemoattractant protein-1 (MCP-1) and JE, belongs to the CC chemokine family. CCL2 is secreted by a wide array of cells including monocytes, macrophages, dendritic cells, endothelial cells, astrocytes, and osteoblasts. In addition to its chemotactic properties, CCL2 is involved in processes of degranulation, upregulation of receptors, cell survival, and secretion of cytokines. Its presence in multiple tissues has been linked to various diseases such as Alzheimer's, diabetes, tuberculosis, osteoporosis, and cancer where CCL2 plays a role in tumor microenvironment.

Alternative namesC-C motif chemokine 2, Chemoattractant Protein 1, MCP-1, MCP1, HC11, GDCF-2, HSMCR30, MCAF, SCYA2, SMC-CF
Cell typeMonocyte/MΦ
Gene ID6347