ELISA Flex: Human CCL4 (HRP)

Standard curve for human CCL4 (MIP-1β) ELISA Flex kit.

ELISA Flex: Human CCL4 (HRP)

3494-1H-6

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You may add these complementary products at a reduced price.

ELISA diluent
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ELISA substrate: TMB for HRP
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This offer is valid when purchasing ELISA Flex: Human CCL4 (HRP) or other qualifying products.

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMTBN13
Detection mAbMTBN3, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant human CCL4 ELISA standard
Buffer/SolutionStandard reconstitution buffer A8

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ELISA diluent

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human CCL4 (MIP-1β) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human CCL4 (HRP)
ApplicationELISA
AnalyteCCL4 (MIP-1β)
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human CCL4 (MIP-1β).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range2-500 pg/ml
Standard curve for human CCL4 (MIP-1β) ELISA Flex kit.

Representative standard curve for human CCL4 (MIP-1β) ELISA Flex kit using TMB substrate.

Datasheet/ProtocolSDS

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CCL4 (MIP-1β)

Analyte description

The CCL4 chemokine plays a role in inflammation and chemotaxis. CCL4 is produced by a variety of cells, including monocytes, NK cells, and T cells. It binds to CCR5. This receptor is expressed by T cells and macrophages (among others). CCL4 is involved in the host response to a wide range of pathogens, it's been especially studied in HIV. Furthermore, dysregulation of CCL4 expression has been associated with various pathological conditions, including autoimmune diseases and cancer. CCL4 is also named macrophage inflammatory protein-1β (MIP-1β). 

Alternative namesC-C motif chemokine ligand 4, CCL4, macrophage inflammatory protein-1 beta, MIP-1β, MIP-1-beta, Act-2, AT744, G-26, HC21, LAG-1, LAG1, SCYA2, SCYA4, ACT2, AT744.1, MIP1B, MIP1B1
Gene ID6351