ELISA Flex: Human EBI3 (ALP)

3459-1A-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MT23A14
Detection mAb	MT140, biotin
Enzyme conjugate	Streptavidin-ALP
Standard	Recombinant human IL-27 ELISA standard
Buffer/Solution	Standard reconstitution buffer

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Delivery 4-9 business days

Shipping $0

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ELISA diluent

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human EBI3 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human EBI3 (ALP)
Application	ELISA
Analyte	EBI3
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human EBI3 subunit of IL-27 and IL-35. The assay will detect both IL-27 and IL-35.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Standard range

15-3000 pg/ml

Representative standard curve for human EBI3 ELISA Flex kit using pNPP substrate.

Documents

Datasheet/Protocol SDS

Human IL-12 family mAb guide

Learn how to combine Mabtech’s antibodies for the detection of human IL-12, IL-12/-23 (p40), IL-23, IL-27, and EBI3 in ELISA and ELISpot.

The IL-12 cytokine family

The IL-12 cytokine family consists of IL-12, IL-23, IL-27, IL-35, and IL-39. The existence of IL-39 in human remains, however, to be confirmed. The proteins are heterodimers of different subunits which are either unique or shared by several family members. IL-12 is a heterodimer, (p70), consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23 which is a heterodimer of p40 and a unique p19 subunit. The p40 subunit also exists as a monomer and homodimer. The EBI3 subunit is shared with IL-27, IL-35, and IL-39.

Mabtech supplies monoclonal antibodies with defined subunit specificities to p40, p35, p19, p28, and EBI3. Since both IL-12 and IL-23 heterodimers contain the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers. The EBI3 assays will detect IL-27, IL-35, and IL-39.

In our IL-12 and IL-23 assays, the same capture antibodies to p40 (MT86/221) are used. Different detection antibodies enable the detection of IL-12 (p70), IL-12/-23 (p40), and IL-23.

Application guide: mAbs to human IL-12, IL-23, IL-27 and EBI3

IL-12 (p70)

Capture mAbs: MT86/221 (p40 specific)

Detection mAb: MT704 (p70 specific)

IL-23

Capture mAbs: MT86/221 (p40 specific)

Detection mAb: MT155 (p19 specific)

IL-27

Capture mAbs: MT2 7/361 (IL-27 and p28 specific)

Detection mAb: MT140 (EBI3 specific)

IL-12/-23 (p40)

Capture mAbs: MT86/221 (p40 specific)

Detection mAb: MT6 18 (p40 specific)

EBI3 (IL-27 and IL-35)

Capture mAbs: M T23A14 (EBI3 specific)

Detection mAb: MT140 (EBI3 specific)

Get inspired!

Make sure to check out how researchers have used Mabtech's IL-23 ELISA, ELISpot, and FluoroSpot kits in everything from infectious disease research to novel immunotherapies. You can find it all in our Publication database.

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Tutorials

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?

You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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Analyte information

EBI3

Analyte description	EBI3 is a protein subunit that forms heterodimers with other proteins in the IL-12 family. IL-27 is a heterodimer composed of the subunits EBI3 and p28. IL-35 is a heterodimer composed of the subunits EBI3 and p35. IL-39 is a heterodimer consisting of the subunits EBI3 and p19. Native IL-39 has not yet been detected in humans. The EBI3 has ca. 30% sequence similarity to IL-12 p40.
Alternative names	Epstein–Barr virus-induced gene3, EBI3, Interleukin-27 subunit beta, IL-27B, IL27B, IL35B
Cell type	Monocyte/MΦ, mDC
Gene ID	10148