ELISA Flex: Human IL-10 (ALP)

ELISA Flex: Human IL‑10 (ALP)

3430-1A-6

Publications: 76

Documents

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	9D7
Detection mAb	12G8, biotin
Enzyme conjugate	Streptavidin-ALP
Standard	Recombinant human IL-10 ELISA standard
Buffer/Solution	Standard reconstitution buffer

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISA diluent

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-10 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human IL-10 (ALP)
Application	ELISA
Analyte	IL-10
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-10.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Standard range

2-200 pg/ml

Representative standard curve for human IL-10 ELISA Flex kit using pNPP substrate.

Documents

Datasheet/Protocol
SDS

Product highlights

Behind the scenes, Published March 27, 2023

We received an award

We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category.

Tutorials

Tutorial, Published July 31, 2023

ELISA publications by sample type

Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Publications (76)

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Analyte information

IL-10

Analyte description	Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.
Alternative names	Interleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell type	T cell, Treg, Monocyte/MΦ, mDC
Gene ID	3586