ELISA Flex: Human IL‑17A/F (ALP)

ELISA Flex: Human IL‑17A/F (ALP)

3523-1A-6

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Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT504
Detection mAbMTF411, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human IL-17A/F ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

Shipping $0

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-17A/F in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human IL-17A/F (ALP)
ApplicationELISA
AnalyteIL-17A/F
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs enabling specific detection of human IL-17A/F.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range25-4000 pg/ml
Representative standard curve for human IL-17F ELISA Flex kit using pNPP substrate.
Datasheet/ProtocolSDS

Selecting the appropriate mAbs is key to effectively detecting human IL-17A/F. This mAb guide describes which combination of our mAbs should be used in ELISA and ELISpot assays.

 

Application guide: ELISA

Our human IL-17A/F ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Coating mAb: MT504, unconjugated
Detection mAb: MTF411, biotin

ELISA IL-17A/F

Application guide: ELISpot

Our human IL-17A/F ELISpot kits can be used to detect the important cytokine directly upon secretion by responding cells.

Coating mAb: MTF411, unconjugated
Detection mAb: MT504, biotin

IL-17A/F ELISpot

 

 

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Featured product, Published February 23, 2022

Broadening our IL‑17 portfolio
Differentiate between human IL-17A, IL17-A/F, and IL-17F with our new monoclonals in ELISA, ELISpot, and FluoroSpot.

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?
You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IL-17A/F

Analyte description

Interleukin 17A/F (IL-17A/F) is a heterodimer composed of an IL-17A subunit and an IL-17F subunit. It is a proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain immune cells of the innate immune system. 

Alternative namesInterleukin 17A/F, IL-17A/F, IL17A/F, CTLA-8, CTLA8, IL-17, IL-17A, IL17, ILA17, CANDF6, IL-17F, IL17A, ML-1, ML1
Cell typeTh17
Gene ID3605, 112744