ELISA Flex: Mouse IgG (HRP)

ELISA Flex: Mouse IgG (HRP)

3825-1HD-6

Publications: 21
Documents
Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

StandardNative mouse IgG ELISA standard
Buffer/SolutionStandard reconstitution buffer
Capture AbAnti-IgG
Detection AbAnti-IgG, HRP

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Delivery 4-9 business days

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IgG in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Mouse IgG (HRP)
ApplicationELISA
AnalyteIgG
ReactivityMouse
Specificity

This kit is based on antibodies enabling detection of mouse IgG.

Cross-reactivity

The assay antibodies may cross-react with Ig from other species.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range0.03-31.6 ng/ml

Representative standard curve for mouse IgG ELISA Flex kit using TMB substrate.

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?
You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IgG1, Igh-4, VH7183, 1810060O09Rik, Igh-1, Igh-1a, IgG2b, Igh-3, gamma2b, Igh-1b, IgG3
Cell typeB cell
Gene ID16017, 380793, 16016, 404711, 380795