ELISA Pro: Mouse IL-4
ELISA Pro: Mouse IL‑4
Components
| Plate | Pre-coated ELISA strip plate (mAb 11B11) |
| Detection mAb | BVD6-24G2, biotin |
| Enzyme conjugate | Streptavidin-HRP |
| Standard | Recombinant mouse IL-4 ELISA standard |
| Substrate | ELISA substrate: TMB for HRP |
| Buffers/Solutions | ELISA diluent |
| Standard reconstitution buffer | |
| Streptavidin-HRP diluent | |
| Wash buffer concentrate | |
| Stop solution | |
| Also provided | Adhesive plate covers |
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Performance
| Standard range | 3.16-1000 pg/ml |
| Sensitivity | 1 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 3 standard deviations to the mean OD of background wells. |
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Analyte information
IL-4
| Analyte description | Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth; the cytokine is essential for B cell secretion of IgE and augments the production of IgG1 in vivo. In addition to regulating B cell growth and immunoglobulin secretion, IL-4 also affects T cells. In vitro, IL-4 promotes T cell growth and can induce cytolytic T cell activity. IL-4 also induces the differentiation of naive CD4 T cells into Th2 cells, which are characterized by their capacity to secrete the cytokines IL-4, IL-5, and IL-10 upon activation, while simultaneously inhibiting the generation of Th1 cells. |
| Alternative names | Interleukin 4, IL-4, IL4, BCGF-1, BSF-1, Il-4 |
| Cell type | T cell, Th2, Treg |
| Gene ID | 16189 |
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