ELISpot Flex: Human IL-17A/F (ALP)

ELISpot Flex: Human IL‑17A/F (ALP)

3523-2A

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMTF411
Detection mAbMT504, biotin
Enzyme conjugateStreptavidin-ALP

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Delivery 4-9 business days

Shipping $0

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ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-17A/F using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-17A/F (ALP)
ApplicationELISpot
AnalyteIL-17A/F
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs enabling specific detection of human IL-17A/F.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBMC were incubated for 48 h in the absence (250,000 cells/well) or presence (50,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-17A/F was analyzed by ELISpot.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to effectively detecting human IL-17A/F. This mAb guide describes which combination of our mAbs should be used in ELISA and ELISpot assays.

 

Application guide: ELISA

Our human IL-17A/F ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Coating mAb: MT504, unconjugated
Detection mAb: MTF411, biotin

ELISA IL-17A/F

Application guide: ELISpot

Our human IL-17A/F ELISpot kits can be used to detect the important cytokine directly upon secretion by responding cells.

Coating mAb: MTF411, unconjugated
Detection mAb: MT504, biotin

IL-17A/F ELISpot

 

 

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IL-17A/F

Analyte description

Interleukin 17A/F (IL-17A/F) is a heterodimer composed of an IL-17A subunit and an IL-17F subunit. It is a proinflammatory cytokine produced by activated Th17 (T helper 17) cells and certain immune cells of the innate immune system. 

Alternative namesInterleukin 17A/F, IL-17A/F, IL17A/F, CANDF6, IL-17F, IL17A, ML-1, ML1, CTLA-8, CTLA8, IL-17, IL-17A, IL17, ILA17
Cell typeTh17
Gene ID112744, 3605