ELISpot Flex: Human IL‑17F (ALP)

ELISpot Flex: Human IL‑17F (ALP)

3522-2A

Publications: 0
Documents
Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMTF1927
Detection mAbMTF912, biotin
Enzyme conjugateStreptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
1 / 2

ELISpot substrate: BCIP/NBT-plus for ALP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-17F using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human IL-17F (ALP)
ApplicationELISpot
AnalyteIL-17F
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-17F.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of PPD and the number of cells secreting IL-17F was analyzed by ELISpot.
Datasheet/ProtocolSDS

Featured product, Published February 23, 2022

Broadening our IL‑17 portfolio
Differentiate between human IL-17A, IL17-A/F, and IL-17F with our new monoclonals in ELISA, ELISpot, and FluoroSpot.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
Loading publications...

IL-17F

Analyte description

Interleukin 17F (IL-17F) is a homodimer composed of two IL-17F subunits. IL17-F is a member of the IL-17A protein family. This proinflammatory cytokine is produced by activated Th17 (T helper 17) cells and certain cells of the innate immune system. Th17 cells play an important role in protection against bacteria and fungi. IL-17F contributes to the pathogenesis of inflammatory and autoimmune diseases, for example, psoriasis.

Alternative namesInterleukin 17F, IL-17F, IL17F, CANDF6, IL17A, ML-1, ML1
Cell typeT cell, Tc, Th17
Gene ID112744