ELISpot Flex: Human TNF‑α (HRP)

Human TNF-α ELISpot. PBMC incubated in the presence or absence of LPS.

ELISpot Flex: Human TNF‑α (HRP)

3512-2H

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This offer is valid when purchasing ELISpot Flex: Human TNF-α (HRP) or other qualifying products.

Publications: 20
Documents
Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT25C5
Detection mAbMT20D9, biotin
Enzyme conjugateStreptavidin-HRP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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Anti-human CD3 mAb (CD3-2), azide free

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Human TNF-α (HRP)
ApplicationELISpot
AnalyteTNF-α
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human TNF-α.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore at 4-8 °C upon receipt.
Shelf lifeAt least 18 months from date of receipt.
Human TNF-α ELISpot. PBMC incubated in the presence or absence of LPS.
Human PBMC were incubated overnight with LPS (8,000 cells/well) or medium only (25,000 cells/well) and the number of cells secreting TNF-α was analyzed by ELISpot.
Datasheet/ProtocolSDS

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Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, DIF-alpha, TNFA, TNFSF2, TNLG1F
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID7124
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ELISpot Plus: Human TNF-α (HRP)