ELISpot Flex: Mouse IgE (ALP)

ELISpot Flex: Mouse IgE (ALP)

3815-2A

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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT56E
Detection mAbMT44E, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
StimuliAnti-CD40 mAb (CD40-A)
Recombinant mouse IL-4

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot plates, white


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgE using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

IgE secretion can be induced by pre-activation of cells with the included anti-CD40 mAb and IL-4. Please note that stimulation with anti-CD40/IL-4 induces switching to IgE and IgG1 and results with stimulated cells may therefore not be representative of the situation in vivo.

Mouse IgE allotypes
Mouse strains with the IgEa allotype include e.g., AKR, BALB/c, C3H/He, and CBA. Mouse strains with the IgEb allotype include e.g., C57BL/6, C57BL/10, and SJL.

Recommendation

Product details

ProductELISpot Flex: Mouse IgE (ALP)
ApplicationELISpot
AnalyteIgE
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IgE, IgEa (allotype a) and IgEb (allotype b).

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

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DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

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Isolation, freezing, and thawing of splenocytes
This is a protocol on how to best (i) isolate, (ii) freeze, and (iii) thaw splenocytes.
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IgE

Analyte description

The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.

Alternative namesImmunoglobulin E, IgE, Gm900
Cell typeB cell
Gene ID380792