ELISpot Flex: Mouse IL‑1β (ALP)

Mouse spleen cells (600,000 cells/well) were incubated overnight in the absence or presence of LPS + IFN-γ and the number of cells secreting IL-1β was analyzed by ELISpot.

ELISpot Flex: Mouse IL‑1β (ALP)

3317-2A

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You may add these complementary products at a reduced price.

ELISpot substrate: BCIP/NBT‑plus for ALP
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ELISpot plates, white
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This offer is valid when purchasing ELISpot Flex: Mouse IL-1β (ALP) or other qualifying products.

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Bulk or custom format
Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMTB52
Detection mAbMTB2433, biotin
Enzyme conjugateStreptavidin-ALP

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Delivery 4-9 business days

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ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IL-1β using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Flex: Mouse IL-1β (ALP)
ApplicationELISpot
AnalyteIL-1β
ReactivityMouse
Specificity

This kit is based on a matched pair of mAbs specific for mouse IL-1β.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Mouse spleen cells (600,000 cells/well) were incubated overnight in the absence or presence of LPS + IFN-γ and the number of cells secreting IL-1β was analyzed by ELISpot.

Mouse spleen cells (600,000 cells/well) were incubated overnight in the absence or presence of LPS + IFN-γ and the number of cells secreting IL-1β was analyzed by ELISpot.

Datasheet/ProtocolSDS

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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
 

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta
Cell typeMonocyte/MΦ, mDC
Gene ID16176