ELISpot Path: Vaccinia Human IFN-γ

ELISpot Path: Vaccinia Human IFN‑γ

3420-4AST-P7-1

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A straightforward setup for functional analysis of a specific scientific question.

Components

PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Detection mAb7-B6-1, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolsVaccinia (CD4), human
Vaccinia (CD8), human

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Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to Vaccinia virus peptides. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition

PepPool: Vaccinia (CD4), human contains 56 defined peptides from 34 vaccinia virus proteins. The peptides are MHC-II restricted. Of the included peptides, 91% share >90% homology with monkeypox.

PepPool: Vaccinia (CD8), human  contains 102 defined peptides from 59 vaccinia virus proteins. The peptides are MHC-I restricted. Of the included peptides, 79% share >90% homology with monkeypox virus.

Recommendation

Product details

ProductELISpot Path: Vaccinia Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. Peptide pools should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

Human PBMC (500,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: Vaccinia (CD4), human (product code 3635-1) and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS.

Datasheet/Protocol
SDS

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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458