ELISpot Plus: Human Granzyme B (ALP)

3486-4APW-2

Publications: 59

Documents

Bulk or custom format

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb MT28)
Detection mAb	MT8610, biotin
Enzyme conjugate	Streptavidin-ALP
Substrate	BCIP/NBT-plus

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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PepPool: CEF (CD8), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human granzyme B using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human Granzyme B (ALP)
Application	ELISpot
Analyte	Granzyme B
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human granzyme B.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

PBMC (250,000 cells/well) were incubated for 48 h in the absence or presence of a mixture of peptide pools: CEFTA peptide pool for human CD4 T cells (product code 3617-1), CEF extended peptide pool for human CD8 T cells (product code 3618-1), and CMV peptide pool for human CD4 and CD8 T cells (product code 3619-1), and the number of cells secreting Granzyme B was analyzed by ELISpot.

Documents

Datasheet/Protocol SDS

Human Granzyme B mAb guide

Selecting the appropriate mAbs is key to effectively detecting human Granzyme B, a critical serine protease secreted by cytotoxic T cells and natural killer cells. This mAb guide describes which combination of our Granzyme B mAbs should be used in different immunoassays.

Application guide: ELISA

Our human granzyme B ELISA kits can be used to detect the important cytokine in solution, whether it be in serum, cell culture media, or plasma samples.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

Application guide: ELISpot

Our human granzyme B ELISpot kits can be used to detect the important cytokine directly upon secretion by T cells.

Capture mAb: MT28, unconjugated
Detection mAb: MT8610, biotin

Application guide: FluoroSpot

Our human granzyme B FluoroSpot kits can be used to detect the important cytokine directly upon secretion by T cells alongside other analytes in the multiplex format.

Capture mAb: MT34B6, unconjugated
Detection mAb: MT28, biotin

Get inspired!

Make sure to check out how researchers have used Mabtech's human Granzyme B ELISA, ELISpot, and FluoroSpot kits in everything from vaccine development to SARS-CoV-2 research. You can find it all in our Publication database.

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (59)

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Analyte information

Granzyme B

Analyte description	Granzyme B is a serine protease released by the cytoplasmic granules within cytotoxic T cells and NK cells. Granzyme B will act together with perforin and other cytotoxic mediators to induce target cell death by apoptosis.
Alternative names	Granzyme B, GzmB, GrB, GrzB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Cell type	T cell, Tc, Th1, NK cell
Gene ID	3002