ELISpot Plus: Human IL‑2 (ALP)

PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PPD and the number of cells secreting IL-2 was analyzed by ELISpot.

ELISpot Plus: Human IL‑2 (ALP)

3445-4APW-2

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You may add these complementary products at a reduced price.

PepPool: CEFTA (CD4), human
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This offer is valid when purchasing ELISpot Plus: Human IL-2 (ALP) or other qualifying products.

Publications: 53
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The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT2A91/2C95)
Detection mAbMT8G10, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
Positive controlAnti-CD3 mAb (CD3-2)

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PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IL-2 (ALP)
ApplicationELISpot
AnalyteIL-2
ReactivityHuman
SpecificityThis kit is based on a matched pair of mAbs specific for human IL-2.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf lifeAt least 18 months from date of receipt.
PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.
PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PPD and the number of cells secreting IL-2 was analyzed by ELISpot.
Datasheet/ProtocolSDS

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IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID3558
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