ELISpot Plus: Human IL‑22 (ALP)

3475-4APW-2

Publications: 12

Documents

Bulk or custom format

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb MT12A3)
Detection mAb	MT7B27, biotin
Enzyme conjugate	Streptavidin-ALP
Substrate	BCIP/NBT-plus
Positive control	Anti-CD3 mAb (CD3-2)

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Complementary products

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PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-22 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human IL-22 (ALP)
Application	ELISpot
Analyte	IL-22
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-22.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

Human PBMC were incubated for 48 h in the absence (500,000 cells/well), or presence (100,000 cells/well) of anti-CD3 monoclonal antibody (available from Mabtech, code 3610-1-50) and the number of cells secreting IL-22 was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (12)

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Analyte information

IL-22

Analyte description	The cytokine interleukin 22 (IL-22) is mainly produced by activated CD4 T helper 17 (Th17) and T helper 22 (Th22) cells. The IL-22 receptor is expressed on non-immune cells, particularly epithelial cells and keratinocytes, and IL-22 promotes innate immune responses versus bacterial infections in these target cells. In addition, Th22 cells have been shown to play a role in the pathophysiology of several human skin diseases.
Alternative names	Interleukin-22, IL-22, IL22, IL-21, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18
Cell type	T cell, Th1, Th17, Tfh
Gene ID	50616