ELISpot Plus: Porcine IL‑2 (ALP)

Porcine PBMC (100,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IL-2 was analyzed by ELISpot.

ELISpot Plus: Porcine IL‑2 (ALP)

3140-4APW-2

$565

Publications: 0
Documents
Bulk or custom format
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAbs MT264/267)
Detection mAbMT265, biotin
Enzyme conjugateStreptavidin-ALP enzyme conjugate for ELISpot
SubstrateBCIP/NBT-plus substrate for ELISpot

Low stock

Delivery within 3-4 weeks

Shipping $0

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting porcine IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Porcine IL-2 (ALP)
ApplicationELISpot
AnalyteIL-2
ReactivityPig
Specificity

This kit is based on a matched pair of mAbs specific for porcine (pig) IL-2.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
Porcine PBMC (100,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IL-2 was analyzed by ELISpot.
Porcine PBMC (100,000 cells/well) were incubated for 20 h in the absence or presence of PHA and the number of cells secreting IL-2 was analyzed by ELISpot.
Datasheet/ProtocolSDS

Tutorial
Published: December 28, 2023 | 2 minute read

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
Loading publications...

IL-2

Analyte description

Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases. 

Alternative namesInterleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, POIL2, TCGF
Cell typeT cell, Th1, Th2, Th17, Tfh
Gene ID396868