Goat anti-mouse IgG, biotin

Goat anti‑mouse IgG, biotin

3825-6-250

Publications: 21
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Content

Goat anti-mouse IgG, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This antibody enables specific detection of mouse IgG in enzyme immunoassays such as indirect ELISA and Western blot.

Serum/Plasma samples

Recommendation

It is recommended to establish the optimal antibody concentration for the assay system used.

Product details

ProductGoat anti-mouse IgG, biotin
ApplicationELISA, Western blot
AnalyteIgG
AntibodyGoat anti-mouse IgG
ConjugateBiotin
ClonalityPolyclonal
Immunogen

Native mouse IgG

HostGoat
ReactivityMouse
Specificity

Mouse IgG H-chains

Cross-reactivity

No cross-reactivity with mouse IgA, IgM, human Ig, or non-immunoglobulin serum proteins. Cross-reacts with rat immunoglobulins and may cross-react with immunoglobulins from other species.

Purification

Purified from in vitro cultures by protein G affinity chromatography.

Biotinylation

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Concentration0.5 mg/ml
Supplied in

PBS with 0.02% sodium azide. Sterile-filtered (0.2 µm).

Contents

Goat anti-mouse IgG, biotinylated. Supplied at 0.5 mg/ml in PBS with 0.02% sodium azide.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.

Shelf lifeAt least 18 months from date of receipt.
Datasheet/Protocol
SDS

Featured product, Published June 8, 2023

Mouse IgG ELISA available with HRP
Our ELISA Flex kit for detecting Mouse IgG is now available in the HRP detection format!
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IgG1, Igh-4, VH7183, 1810060O09Rik, Igh-1, Igh-1a, IgG2b, Igh-3, gamma2b, Igh-1b, IgG3
Cell typeB cell
Gene ID16017, 380793, 16016, 404711, 380795