ELISA Flex: Human Granzyme A (ALP)

ELISA Flex: Human Granzyme A (ALP)

3484-1A-6

Publications: 4
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbGA29
Detection mAbGA28, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human granzyme A ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human granzyme A in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human Granzyme A (ALP)
ApplicationELISA
AnalyteGranzyme A
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human granzyme A.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range125-12500 pg/ml
Representative standard curve for human Granzyme A ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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Granzyme A

Analyte description

Granzyme A is a serine protease packed together with e.g., granzyme B and perforin in cytoplasmic granules within cytotoxic T cells and NK cells. Release of the granule content is crucial for the elimination of infected or transformed cells. Perforin facilitates the delivery of granzymes, and cells resistant to the cytotoxic action of granzyme B may be sensitive to granzyme A and vice versa. Thus, the content of the granules will act in concert to induce target cell death by apoptosis.

Alternative namesGranzyme A, GzmA, GrA, GrzA, CTLA3, HFSP
Cell typeT cell, Tc, Th1, Th17, NK cell
Gene ID3001