ELISpot Plus: Human IL‑10 (HRP)

3430-4HPW-2

Publications: 59

Documents

Bulk or custom format

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb 9D7)
Detection mAb	12G8, biotin
Enzyme conjugate	Streptavidin-HRP
Substrate	ELISpot substrate: TMB for HRP
Positive control	Anti-CD3 mAb (CD3-2)

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Delivery 4-9 business days

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Complementary products

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PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-10 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human IL-10 (HRP)
Application	ELISpot
Analyte	IL-10
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-10.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

PBMC (10,000 cells/well) were incubated for 20 h in the absence or presence of LPS and the number of cells secreting IL-10 was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (59)

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Analyte information

IL-10

Analyte description	Interleukin 10 (IL-10) is a pleiotropic cytokine produced by activated T cells and monocytes. It can modulate the functions of immune responses in both a stimulatory and an inhibitory way. IL-10 is thereby involved in the regulation of immune reactions and inflammatory responses.
Alternative names	Interleukin-10, IL-10, IL10, CSIF, GVHDS, IL-10A, TGIF
Cell type	T cell, Treg, Monocyte/MΦ, mDC
Gene ID	3586