ELISA Flex: Human IL‑12 p70 (ALP)

ELISA Flex: Human IL‑12 p70 (ALP)

3455-1A-6

Publications: 32
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbMT86/221
Detection mAbMT704, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human IL-12 p70 ELISA standard (lyophilized)
Buffer/SolutionStandard reconstitution buffer

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-12 p70 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma and can also be present in serum/plasma from other species. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human IL-12 p70 (ALP)
ApplicationELISA
AnalyteIL-12 (p70)
ReactivityHuman, Non-human primates
Specificity

This kit is based on a matched pair of mAbs specific for human IL-12 p70.

Cross-reactivity

The mAbs react with human IL-12p70 and cross-react with IL-12 p70 from non-human primates (NHP). For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range6-600 pg/ml
Representative standard curve for human IL-12 (p70) ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Learn how to combine Mabtech’s antibodies for the detection of human IL-12, IL-12/-23 (p40), IL-23, IL-27, and EBI3 in ELISA and ELISpot.

 

The IL-12 cytokine family

The IL-12 cytokine family consists of IL-12, IL-23, IL-27, IL-35, and IL-39. The existence of IL-39 in human remains, however, to be confirmed. The proteins are heterodimers of different subunits which are either unique or shared by several family members. IL-12 is a heterodimer, (p70), consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23 which is a heterodimer of p40 and a unique p19 subunit. The p40 subunit also exists as a monomer and homodimer. The EBI3 subunit is shared with IL-27, IL-35, and IL-39.

Mabtech supplies monoclonal antibodies with defined subunit specificities to p40, p35, p19, p28, and EBI3. Since both IL-12 and IL-23 heterodimers contain the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers. The EBI3 assays will detect IL-27, IL-35, and IL-39.

In our IL-12 and IL-23 assays, the same capture antibodies to p40 (MT86/221) are used. Different detection antibodies enable the detection of IL-12 (p70), IL-12/-23 (p40), and IL-23.

 

Application guide: mAbs to human IL-12, IL-23, IL-27 and EBI3

 

 IL-12 (p70) 
 
 
Capture mAbs: MT86/221 (p40 specific)
 
Detection mAb: MT704 (p70 specific)

     

 IL-23 
 
 
Capture mAbs: MT86/221 (p40 specific)
 
Detection mAb: MT155 (p19 specific)

     

 IL-27 
 
 
Capture mAbs: MT27/361 (IL-27 and p28 specific)
 
Detection mAb: MT140 (EBI3 specific)
 IL-12/-23 (p40) 
 
 
Capture mAbs: MT86/221 (p40 specific)
 
Detection mAb: MT618 (p40 specific)

     

 EBI3 (IL-27 and IL-35) 
 
Capture mAbs: MT23A14 (EBI3 specific)
 
Detection mAb: MT140 (EBI3 specific)

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's IL-23 ELISA, ELISpot, and FluoroSpot kits in everything from infectious disease research to novel immunotherapies. You can find it all in our Publication database.

The systems reactive with NHPs are either based on cross-reactive human kits or specifically developed monkey kits (NHP).

Cross-reactivity verification tests have been performed in ELISpot and/or ELISA by Mabtech and/or by others. Evaluations of assays with less solid evidence of cross-reactivity are shown as symbols within parentheses.

 Rhesus MacaqueCynomolgus MacaquePig-tailed MacaqueBaboon*Sooty MangabeyAfrican Green MonkeyNight Monkey*Common MarmosetSquirrel Monkey
ASSAYOld worldNew world
ApoA1      
ApoB       
ApoE (NHP)       
ApoE       
ApoH       
CCL2 (MCP-1)       
CCL4 (MIP-1β) ( ) ( )( )( )( )
CCL22 (MDC) ( ) ( )( )( )( )
CD25       
GM-CSF( )( )   
Granzyme A        
Granzyme B (NHP)       
Granzyme B     
IFN-α2        
IFN-α pan   ( )   
IFN-γ (NHP)
IFN-γ( )
IgA (NHP)       
IgA       
IgG       
IgM       
INS     
IL-1α        
IL-1β        
IL-2 (NHP)
IL-3       
IL-4( )( )( )( )
IL-5( )( )( ) 
IL-6( )( ) ( ) 
IL-8 (NHP)        
IL-8 (ELISA)        
IL-8 (ELISpot)        
IL-10 (NHP)      
IL-10       
IL-12/-23 (p40)      
IL-12 (p70)        
IL-13 (NHP)( )( )( )  
IL-13        
IL-17A (NHP)( )( )   
IL-17A     
IL-17F        
IL-17A/F        
IL-21      
IL-22        
IL-23     
IL-27        
IL-31        
IP-10       
Perforin( )   
TGF-β1 (latent)       
TNF-α (NHP)( )  ( )( )( )( )
TNF-α       
          
SINGLE mAbs         
Cell stimulation         
CD3, mAb CD3-1       
CD3, mAb CD3-2       
CD28, mAb CD28-A       
Flow cytometry          
IFN-γ mAb 1-D1K( )( )( )( )( )( )
IL-2 mAb MT8G10( )( )( )( ) ( )( )( )
IL-4 mAb IL4-3( )       
IL-17A mAb MT504        
Perforin mAb Pf-344     
TNF-α mAb MT15B15       
Ig reagents         
IgG1 mAb MTG1218       
IgG1 mAb MT1939       
IgG2 mAb H6200       
IgG2 mAb MTG211E       
IgG3 mAb MTG34       
IgG4 mAb MTG42       
Western blot         
IFN-γ mAb MT111W( )( )( )( )( )( )( )( )( )
TNF-α mAb MT15B15( )( )( )  ( )( )( )( )

Symbol keys:


Cross-reactivity

Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA.

  • good
  • poor
  • no

Indications of Cross-reactivity

Based on reports from others or by analysis using recombinant proteins.

  • ( ) good
  • ( ) poor
  • ( ) no

Monkey kits

Specifically developed monkey kits are marked with (NHP).

Blank boxes

Represent cross-reactivities not evaluated.

* Comprises several species. Cross-reactivity may have to be verified on a species basis.

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?
You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IL-12 (p70)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Alternative namesInterleukin 12, IL-12, IL12, CLMF, IL-12A, NFSK, NKSF1, P35, CLMF2, IL-12B, IMD28, IMD29, NKSF, NKSF2
Cell typeMonocyte/MΦ, mDC
Gene ID3592, 3593
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ELISA Pro: Human IL-12 p70