ELISpot Plus: Human IL-2 (HRP)

ELISpot Plus: Human IL‑2 (HRP)

3445-4HPW-2

Publications: 53

Documents

Questions?

The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAbs MT2A91/2C95)
Detection mAb	MT8G10, biotin
Enzyme conjugate	Streptavidin-HRP
Substrate	ELISpot substrate: TMB for HRP
Positive control	Anti-CD3 mAb (CD3-2)

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human IL-2 (HRP)
Application	ELISpot
Analyte	IL-2
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-2.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

PBMC were incubated for 20 h in the absence (50,000 cells/well) or presence (25,000 cells/well) of anti-CD3 mAb + anti-CD28 mAb and the number of cells secreting IL-2 was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot

An in-depth guide to how ELISpot works, including best practice and tips and tricks.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Product highlights

Highlighted research, Published October 11, 2024

Immunoassays for vaccine research

Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.

Publications (53)

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Analyte information

IL-2

Analyte description	Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases.
Alternative names	Interleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell type	T cell, Th1, Th2, Th17, Tfh
Gene ID	3558