ELISA Flex: Human IL-3 (ALP)

ELISA Flex: Human IL‑3 (ALP)

3435-1A-6

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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbIL3A
Detection mAbIL3B, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant human IL-3 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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Delivery 4-9 business days

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ELISA substrate: pNPP for ALP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IL-3 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human IL-3 (ALP)
ApplicationELISA
AnalyteIL-3
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-3.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range25-2500 pg/ml
Representative standard curve for human IL-3 ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IL-3

Analyte description

The cytokine interleukin 3 (IL-3) is mainly produced by activated T cells. IL-3 is also called multi-lineage-colony stimulating factor since it induces the growth and differentiation of many cells. IL-3 also activates mast cells, eosinophils, basophils, and macrophages.

Alternative namesInterleukin 3, IL-3, IL3, colony-stimulating factor, multi-CSF, mast cell growth factor, MULTI-CSF, MCGF
Cell typeT cell, Th2
Gene ID3562