ELISpot Plus: Human IL-3 (ALP)

ELISpot Plus: Human IL‑3 (ALP)

3435-4APW-2

Publications: 0

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The easiest way to achieve reproducibility in ELISpot.

Components

Plate	Pre-coated ELISpot plate, white MSIP (mAb IL3A)
Detection mAb	IL3B, biotin
Enzyme conjugate	Streptavidin-ALP
Substrate	BCIP/NBT-plus
Positive control	Anti-CD3 mAb (CD3-2)

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Delivery 4-9 business days

Shipping $0

Complementary products

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PepPool: CEFTA (CD4), human

Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-3 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Plus: Human IL-3 (ALP)
Application	ELISpot
Analyte	IL-3
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-3.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.
Shelf life	At least 18 months from date of receipt.

Performance

Human PBMC were incubated for 48 hours in an ELISpot plate. Cells were unstimulated (250,000 cells/well) or stimulated with either a mixture of Tetanus toxoid (TT) and anti-CD28 antibody (250,000 cells/well), or PMA/ionomycin (50,000 cells/well). The number of cells secreting IL-3 was analyzed by ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot

An in-depth guide to how ELISpot works, including best practice and tips and tricks.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (0)

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Analyte information

IL-3

Analyte description	The cytokine interleukin 3 (IL-3) is mainly produced by activated T cells. IL-3 is also called multi-lineage-colony stimulating factor since it induces the growth and differentiation of many cells. IL-3 also activates mast cells, eosinophils, basophils, and macrophages.
Alternative names	Interleukin 3, IL-3, IL3, colony-stimulating factor, multi-CSF, mast cell growth factor, MULTI-CSF, MCGF
Cell type	T cell, Th2
Gene ID	3562