Isotype control mAb (97B4) for mouse IgG2a, PF488P

3613-71-100T

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Content

Monoclonal antibody 97B4, conjugated to PF488P fluorophore. Ready to use.

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Product specifications

Intended use

This monoclonal antibody is recommended as mouse IgG2a isotype control in flow cytometry. It has been used as isotype control with cells from different species.

Serum/Plasma samples

Recommendation

5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF488P is excited by the blue laser (488 nm). The excitation max is 490 nm and the emission max is 516 nm.

Product details

Product	Isotype control mAb (97B4) for mouse IgG2a, PF488P
Application	Flow cytometry, Isotype control
Analyte	Non-specific
Antibody	97B4
Conjugate	PF488P
Clonality	Monoclonal
Immunogen	Bacterial antigen
Host	Mouse
Isotype	IgG2a
Specificity	Bacterial protein
Supplied in	PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm).
Contents	Monoclonal antibody 97B4, conjugated to PF488P fluorophore. Ready to use.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store product at 4-8 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Profile of equine lymphocytes stained with mouse IgG2a isotype control antibody 97B4 (dashed line) and anti-equine IFN-γ antibody (solid line). Cells were fixed and permeabilized prior to staining. The histogram derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.

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Analyte information

Non-specific

Analyte description

Isotype controls are often used as negative controls in flow cytometry, immunochemistry, or Western blot experiments. An isotype control is an antibody that doesn't bind the target analyte but otherwise matches the properties of the primary antibody. For example, an isotype control should be conjugated in the same way and match the isotype of the target-specific antibody used in the assay.