ELISA Flex: Mouse IL-12 p70 (HRP)

ELISA Flex: Mouse IL‑12 p70 (HRP)

3456-1H-6

Publications: 21
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbC17.8
Detection mAbIL12-IV, biotin
Enzyme conjugateStreptavidin-HRP
StandardRecombinant mouse IL-12 p70 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA substrate: TMB for HRP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IL-12 p70 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Mouse IL-12 p70 (HRP)
ApplicationELISA
AnalyteIL-12 (p70)
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for mouse IL-12 p70.

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range40-4000 pg/ml
Representative standard curve for mouse IL-12 (p70) ELISA Flex kit using TMB substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

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Tutorial, Published July 31, 2023

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Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

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Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IL-12 (p70)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. The p40 subunit is shared with IL-23. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Alternative namesInterleukin 12, IL-12, IL12, IL-12p35, Il-12a, Ll12a, p35, Il-12b, Il-12p40, Il12p40, p40
Cell typeMonocyte/MΦ, mDC
Gene ID16159, 16160
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ELISA Pro: Mouse IL-12 p70