ELISA Flex: Mouse IL‑12/‑23 p40 (ALP)

ELISA Flex: Mouse IL‑12/‑23 p40 (ALP)

3451-1A-6

Publications: 5
Documents
Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAbC15.6
Detection mAbC17.8, biotin
Enzyme conjugateStreptavidin-ALP
StandardRecombinant mouse IL-12 p70 ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA substrate: pNPP for ALP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IL-12/-23 p40 in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Mouse IL-12/-23 p40 (ALP)
ApplicationELISA
AnalyteIL-12/-23 (p40)
ReactivityMouse
SpecificityThis kit is based on a matched pair of mAbs specific for the p40 subunit of mouse IL-12 and IL-23. The antibodies recognize p40 monomer and homodimer, IL-12 p70 (p40+p35), and IL-23 (p40+p19).

Shipping and Storage

ShippingShipped at ambient temperature.
StorageStore antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf lifeAt least 18 months from date of receipt.
Standard range40-4000 pg/ml
Representative standard curve for mouse IL-12/-23 (p40) ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?
You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.
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IL-12/-23 (p40)

Analyte description

Interleukin 12 (IL-12) is, when biologically active, a heterodimer (p70) consisting of two covalently linked subunits, p35 and p40. IL-12 is produced by antigen-presenting cells, specifically dendritic cells, and macrophages. IL-12 is a proinflammatory cytokine that promotes Th1-type responses by inducing IFN-γ production and enhancing the proliferation and cytotoxicity of NK and T cells.

Interleukin-23 (IL-23) is a disulfide-linked heterodimer that consists of a unique p19 subunit and the p40 subunit of IL-12. IL-23 is a proinflammatory cytokine, which is produced by macrophages and dendritic cells upon infections with certain pathogens. The IL-23 receptor is expressed on Th17 cells, and IL-23 is responsible for Th17 expansion and stabilization.

Since both IL-12 and IL-23 heterodimers comprise the p40 subunit, capture immunoassays based on antibodies to p40 will detect both cytokines. Such assays can also detect p40 monomers and dimers.

Alternative namesInterleukin 12, IL-12, IL12, IL12A, IL12B, Il-12b, Il-12p40, Il12p40, p40
Cell typeMonocyte/MΦ, mDC
Gene ID16160
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ELISA Pro: Mouse IL-12/-23 p40