ELISpot

The enzyme-linked immunospot (ELISpot) assay is an extremely sensitive immunoassay performed in a 96-well plate format to quantify protein-secreting cells. 

The assay principle is straight forward: cells are cultured and stimulated inside a plate coated with capture antibody. Cytokines, immunoglobulins, or other target proteins secreted by the cells are captured immediately upon secretion and throughout the stimulation process. After cell removal, secreted proteins are identified using a detection antibody. Visible spots form after adding a precipitating substrate. Each spot corresponds to an individual analyte-secreting cell.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot
An in-depth guide to ELISpot, including best practice and tips and tricks.

Behind the scenes, Published March 30, 2023

Webinar recording: How to succeed with ELISpot
Watch the recording of our ELISpot webinar and make your next experiment a success.

Featured product, Published October 30, 2023

Our ELISpot products
If one cell responds, you will find it. ELISpot is a cell-based immunoassay with detection levels as low as one cell in a million.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published February 19, 2024

Understanding background spots in negative controls of ELISpot and FluoroSpot
Discover why spots might appear in your negative or background control wells during ELISpot and FluoroSpot and how to interpret these results correctly.

Technote, Published November 21, 2023

Technote: Whole Blood ELISpot
Learn how our R&D team developed and optimized a new protocol for using whole blood in the ELISpot assay.

Tutorial, Published March 8, 2023

Using adherent cells in ELISpot and FluoroSpot
Does your ELISpot/FluoroSpot setup include adherent cells? Read up on how to update your protocol to accommodate these cells!