ELISA Flex: Human CCL4 (ALP)

3494-1A-6

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Bulk or custom format

An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb	MTBN13
Detection mAb	MTBN3, biotin
Enzyme conjugate	Streptavidin-ALP
Standard	Recombinant human CCL4 ELISA standard
Buffer/Solution	Standard reconstitution buffer A8

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Delivery 4-9 business days

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ELISA diluent

Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human CCL4 (MIP-1β) in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

Product	ELISA Flex: Human CCL4 (ALP)
Application	ELISA
Analyte	CCL4 (MIP-1β)
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human CCL4 (MIP-1β).

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.
Shelf life	At least 18 months from date of receipt.

Performance

Standard range

2-500 pg/ml

Representative standard curve for human CCL4 (MIP-1β) ELISA Flex kit using pNPP substrate.

Documents

Datasheet/Protocol SDS

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Step-by-step guide to ELISA

Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

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Analyte information

CCL4 (MIP-1β)

Analyte description	The CCL4 chemokine plays a role in inflammation and chemotaxis. CCL4 is produced by a variety of cells, including monocytes, NK cells, and T cells. It binds to CCR5. This receptor is expressed by T cells and macrophages (among others). CCL4 is involved in the host response to a wide range of pathogens, it's been especially studied in HIV. Furthermore, dysregulation of CCL4 expression has been associated with various pathological conditions, including autoimmune diseases and cancer. CCL4 is also named macrophage inflammatory protein-1β (MIP-1β).
Alternative names	C-C motif chemokine ligand 4, CCL4, macrophage inflammatory protein-1 beta, MIP-1β, MIP-1-beta, Act-2, AT744, G-26, HC21, LAG-1, LAG1, SCYA2, SCYA4, ACT2, AT744.1, MIP1B, MIP1B1
Gene ID	6351