ELISA Flex: Human IgE (ALP)

ELISA Flex: Human IgE (ALP)

3810-1A-6

Publications: 17
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Capture mAb107
Detection mAb182, biotin
Enzyme conjugateStreptavidin-ALP
StandardNative human IgE ELISA standard
Buffer/SolutionStandard reconstitution buffer

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ELISA diluent


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of human IgE in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Analysis of serum/plasma with this kit requires the use of ELISA diluent. The ELISA diluent blocks heterophilic antibodies from cross-linking the assay antibodies, thereby preventing false-positive read-outs. Heterophilic antibodies are commonly found in human serum/plasma. The ELISA diluent has been validated using serum/plasma samples from healthy human blood donors. It should be used for dilution of standard, samples, and detection antibody.

Recommendation

Product details

ProductELISA Flex: Human IgE (ALP)
ApplicationELISA
AnalyteIgE
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IgE.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range0.3-30 ng/ml
Representative standard curve for human IgE ELISA Flex kit using pNPP substrate.
Datasheet/Protocol
SDS

Selecting the appropriate mAbs is key to detecting both total and antigen-specific human IgA. This blog post explores Mabtech’s mAbs, tailored for ELISpot to detect human IgA antibody-secreting cells.

 

Application guide: ELISA

 

Antigen-specific IgE

Coating:
Antigen
Detection mAbs:
107/182/101, biotin

IgE Ag

Total IgE

Coating:
107, unconjugated
Detection mAbs:
182, biotin

IgE total

 

Application guide: ELISpot

 

Total IgE

Coating mAb:
107, unconjugated
Detection mAbs:
107/182/101, biotin

ELISpot IgE

 

 

Get inspired!

Make sure to check out how researchers have used Mabtech's human IgE ELISA, ELISpot, and FluoroSpot kits in allergy research. You can find it all in our Publication database.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

How to detect immunoglobulins with ELISA
Detect and quantify immunoglobulins in solution, either antigen-specific or even total Ig.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 
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IgE

Analyte description

The main physiological function of immunoglobulin E (IgE) is to protect against parasitic infestation. However, IgE is also associated with allergy, and allergen-specific IgE antibodies may trigger allergic reactions in the presence of the allergen. IgE is normally found in low amounts in serum/plasma but is significantly increased in allergic individuals, and the detection of allergen-specific IgE serves as the basis for many in vitro assays for allergy diagnosis.

Alternative namesImmunoglobulin E, IgE
Cell typeB cell
Gene ID3497
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ELISA Pro: Human IgE