ELISA Flex: Mouse IgG (ALP)

ELISA Flex: Mouse IgG (ALP)

3825-1AD-6

Publications: 21
Documents
Bulk or custom format
An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Detection mAbAnti-IgG, ALP
StandardNative mouse IgG ELISA standard
Buffer/SolutionStandard reconstitution buffer
Capture AbAnti-IgG

In stock

Delivery 4-9 business days

Shipping $0

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ELISA substrate: pNPP for ALP


Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IgG in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Mouse IgG (ALP)
ApplicationELISA
AnalyteIgG
ReactivityMouse
Specificity

This kit is based on antibodies enabling detection of mouse IgG.

Cross-reactivity

The assay antibodies may cross-react with Ig from other species. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range0.1-100 ng/ml

Representative standard curve for mouse IgG ELISA Flex kit using pNPP substrate.

Datasheet/Protocol
SDS

Tutorial, Published September 20, 2023

ELISA kit with HRP or ALP – what to choose?
You are interested in one of our ELISA Flex kits but wondering whether to choose the kit based on ALP or HRP? It’s a matter of taste, but we'd say HRP is mainly used for the colorimetric reaction. Find out why.

Tutorial, Published July 31, 2023

ELISA publications by sample type
Find out how researchers have used our ELISAs to analyze different types of samples.

Tutorial, Published November 28, 2022

How to detect immunoglobulins with ELISA
Detect and quantify immunoglobulins in solution, either antigen-specific or even total Ig.

Tutorial, Published November 28, 2022

Step-by-step guide to ELISA
Learn how to successfully run your next ELISA experiment with our step-by-step guide to the ELISA protocol.

Featured product, Published June 8, 2023

Mouse IgG ELISA available with HRP
Our ELISA Flex kit for detecting Mouse IgG is now available in the HRP detection format!

Behind the scenes, Published March 27, 2023

We received an award
We are humbled to learn that we received an award from CiteAb. Based on citations for our ELISA kits, Mabtech has been highly commended in the 'ELISA Kit Supplier to Watch in 2023’ category. 
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IgG1, Igh-4, VH7183, 1810060O09Rik, Igh-1, Igh-1a, IgG2b, Igh-3, gamma2b, Igh-1b, IgG3
Cell typeB cell
Gene ID16017, 380793, 16016, 404711, 380795