ELISpot Path: BKV Human IFN-γ

ELISpot Path: BKV Human IFN‑γ

3420-4AST-P9-1

Publications: 0
Documents
Questions?
A straightforward setup for functional analysis of a specific scientific question.

Components

PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Detection mAb7-B6-1, biotin
Enzyme conjugateStreptavidin-ALP
SubstrateBCIP/NBT-plus
Positive controlAnti-CD3 mAb (CD3-2)
Peptide poolBKV (CD4 and CD8), human

In stock

Delivery 4-9 business days

Shipping $0

Product specifications

Intended use

This ELISpot Path kit is intended for the enumeration of cells secreting human IFN-γ in response to BK virus (BKV) peptides. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Peptide pool composition
PepPool: BKV (CD4 and CD8), human contains 65 synthetic peptides derived from the BKV large T protein, small T protein, and VP1 protein. 50 of the peptides are MHC class I-restricted and 15 are MHC class II-restricted. The peptide pool is designed to stimulate T cells with a broad array of HLA class I supertypes. Sequence similarity to corresponding JC virus (JCV) peptides ranges from 60% to 100%, with a median sequence similarity of 80%.

Recommendation

Product details

ProductELISpot Path: BKV Human IFN-γ
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C. The peptide pool should be stored at -20 °C or below. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

Human PBMC (250,000 cells/well) were incubated for 20 h in the absence or presence of PepPool: BKV (CD4 + CD8), human, and the number of cells secreting IFN-γ was analyzed by ELISpot. Analysis was done with Mabtech IRIS. 

Datasheet/Protocol
SDS

Highlighted research, Published October 28, 2024

Immunoassays for infectious disease research
Explore the role of immunoassays like ELISpot and FluoroSpot in infectious disease research. Discover how Mabtech's immunoassays enable researchers to study immune responses at the cellular level for diseases like TB, SARS-CoV-2, Malaria, HIV, and more.

Highlighted research, Published October 28, 2024

T cell therapy treats BKV in transplant patients
This study demonstrates the success of virus-specific T cell therapy in treating BK virus (BKV) infections in immunocompromised transplant patients. ELISpot confirmed the expansion of BKV-specific T cells, with high response rates and no adverse effects, highlighting the therapy's potential.

Featured product, Published December 18, 2023

Study DENV, TBEV, BKV, and Vaccinia with ELISpot
All-in-one kit to answer your research question!
Loading publications...

IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458