ELISpot Plus: Human IFN-γ (HRP)

ELISpot Plus: Human IFN‑γ (HRP)

3420-4HST-2

Publications: 1832
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, strip (mAb 1-D1K)
Detection mAb7-B6-1, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP
Positive controlAnti-CD3 mAb (CD3-2)

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Delivery 4-9 business days

Shipping $0

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PepPool: CEF (CD8), human


Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IFN-γ (HRP)
ApplicationELISpot
AnalyteIFN-γ
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IFN-γ.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC (62,500 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 monoclonal antibody (available from Mabtech, code 3605-1-50) and the number of cells secreting IFN-γ was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot
An in-depth guide to how ELISpot works, including best practice and tips and tricks.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Highlighted research, Published October 11, 2024

Immunoassays for vaccine research
Explore the critical role of immunoassays like ELISpot and FluoroSpot in vaccine development, from evaluating immune responses to tracking vaccine efficacy and potency. These assays provide sensitive, single-cell insights for advancing research and development of viral, bacterial, and cancer vaccines.

Highlighted research, Published July 28, 2020

ELISpot important in evaluating SARS-CoV-2 vaccine efficacy
Two studies that utilized Mabtech's ELISpot IFN-γ kits to evaluate spike protein-specific T cell responses pre- and post-vaccination.

Highlighted research, Published June 30, 2020

T cell immunity in COVID-19
Much emphasis has been put on antibody responses, but also T cells are important in the context of COVID-19. In this study, the authors characterized SARS-CoV-2-specific T cell responses by using an extensive FACS panel, peptide stimulations, tetramer staining, and Mabtech IFN-γ ELISpot.

Highlighted research, Published May 11, 2020

Longevity of immunity against SARS-CoV-2
Investigation of specific T cell responses to SARS-CoV 11 years after outbreak using Mabtech human IFN-γ ELISpot.

Highlighted research, Published November 4, 2019

Potency of cockroach allergens evaluated with FluoroSpot
Immunogenicity of German cockroach extracts on PBMCs from allergic and non-allergic patients, were analyzed with IFN-γ/IL-10/IL-5 FluoroSpot. The assay allowed to study the effect of extracts on T-cell activity.

Highlighted research, Published June 13, 2019

Mabtech IRIS used in MS research
Investigating myelin oligodendrocyte (MOG) antigen with Mabtech FluoroSpot Plus: IFN-γ, IL-22, and IL-17A.

Highlighted research, Published December 10, 2018

2018 Nobel laureate used Mabtech ELISpot
Nobel laureate Professor James P. Allison has been using Mabtech ELISpot: human IFN-γ throughout his research career.

Highlighted research, Published September 20, 2018

ELISpot instrumental in finding breast cancer-reactive T cell clones
The authors sequenced breast cancer lesion for somatic mutations, and subsequently screened TILs for reactivity to identify neoantigens using Mabtech IFN-γ ELISpot.
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IFN-γ

Analyte description

Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Alternative namesInterferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69
Cell typeT cell, Tc, Th1, NK cell
Gene ID3458
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FluoroSpot Plus: Human IFN-γ/IL-2