ELISpot Plus: Human IL-1β (HRP)

ELISpot Plus: Human IL‑1β (HRP)

3416-4HPW-2

Publications: 3
Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

PlatePre-coated ELISpot plate, white MSIP (mAb MT175)
Detection mAb7P10, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting human IL-1β using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Human IL-1β (HRP)
ApplicationELISpot
AnalyteIL-1β
ReactivityHuman
Specificity

This kit is based on a matched pair of mAbs specific for human IL-1β (pro-form and active form).

Cross-reactivity

The mAbs do not cross-react with human IL-1α. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.
PBMC were incubated for 20 h in the presence (8000 cells/well) or absence (25,000 cells/well) of R848 (1μg/ml), and the number of cells secreting IL-1β was analyzed by ELISpot.
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot
We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published September 7, 2023

Step-by-step guide to ELISpot
An in-depth guide to how ELISpot works, including best practice and tips and tricks.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs
This is a protocol on how to best isolate, freeze, and thaw PBMCs.
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IL-1β

Analyte description

Interleukin 1ß (IL-1ß) is a proinflammatory cytokine and inducer of acute phase responses. IL-1ß is produced primarily by monocytes, macrophages, and dendritic cells after induction by microbes.
 

Alternative namesInterleukin-1ß, IL-1ß, IL-1F2, Interleukin-1beta, IL-1 beta, IL1b, Interleukin-1 beta, IL-1, IL1-BETA, IL1F2, IL1beta
Cell typeMonocyte/MΦ, mDC
Gene ID3553
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FluoroSpot Plus: Human IL-1β/IL-6/TNF-α