ELISA Flex: Mouse IgG (ALP)

ELISA Flex: Mouse IgG (ALP)

3825-1AD-6

Publications: 21
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An ELISA designed to detect the native protein – in our most adaptable kit format to match your unique setup. Combine it with your preferred substrate and buffer.

Components

Detection mAbAnti-IgG, ALP
StandardNative mouse IgG ELISA standard
Buffer/SolutionStandard reconstitution buffer
Capture AbAnti-IgG

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Product specifications

Intended use

This ELISA Flex kit is intended for the quantification of mouse IgG in solution. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISA Flex: Mouse IgG (ALP)
ApplicationELISA
AnalyteIgG
ReactivityMouse
Specificity

This kit is based on antibodies enabling detection of mouse IgG.

Cross-reactivity

The assay antibodies may cross-react with Ig from other species. For more information please check the Cross-reactivity guide section below.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies and enzyme conjugate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C.

Shelf lifeAt least 18 months from date of receipt.
Standard range0.1-100 ng/ml

Representative standard curve for mouse IgG ELISA Flex kit using pNPP substrate.

Tutorial, Published July 31, 2023

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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG, IgG1, Igh-4, VH7183, 1810060O09Rik, Igh-1, Igh-1a, IgG2b, Igh-3, gamma2b, Igh-1b, IgG3
Cell typeB cell
Gene ID16017, 380793, 16016, 404711, 380795