ELISpot Flex: Human IL‑2 (ALP)

3445-2A

Publications: 53

Documents

Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT2A91/2C95
Detection mAb	MT8G10, biotin
Enzyme conjugate	Streptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

1 / 4

ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Human IL-2 (ALP)
Application	ELISpot
Analyte	IL-2
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-2.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Performance

PBMC (50,000 cells/well) were incubated for 20 h in the absence or presence of anti-CD3 mAb (available from Mabtech, code 3605-1-50) and the number of cells secreting IL-2 was analyzed by ELISpot.

Documents

Datasheet/Protocol SDS

Product highlights

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Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (53)

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Analyte information

IL-2

Analyte description	Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases.
Alternative names	Interleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF, lymphokine
Cell type	T cell, Th1, Th2, Th17, Tfh
Gene ID	3558