ELISpot Flex: Human IL‑3 (ALP)

3435-2A

Publications: 0

Documents

Bulk or custom format

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	IL3A
Detection mAb	IL3B, biotin
Enzyme conjugate	Streptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

1 / 4

ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting human IL-3 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Human IL-3 (ALP)
Application	ELISpot
Analyte	IL-3
Reactivity	Human
Specificity	This kit is based on a matched pair of mAbs specific for human IL-3.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Performance

Human PBMC were incubated for 48 hours in an ELISpot plate. Cells were unstimulated (250,000 cells/well) or stimulated with either a mixture of Tetanus toxoid (TT) and anti-CD28 antibody (250,000 cells/well), or PMA/ionomycin (50,000 cells/well). The number of cells secreting IL-3 was analyzed by ELISpot.

Documents

Datasheet/Protocol SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 10, 2022

Step-by-step guide to ELISpot

An in-depth guide to ELISpot, including best practice and tips and tricks.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Tutorial, Published September 2, 2022

Isolation, freezing, and thawing of PBMCs

This is a protocol on how to best isolate, freeze, and thaw PBMCs.

Publications (?)

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Analyte information

IL-3

Analyte description	The cytokine interleukin 3 (IL-3) is mainly produced by activated T cells. IL-3 is also called multi-lineage-colony stimulating factor since it induces the growth and differentiation of many cells. IL-3 also activates mast cells, eosinophils, basophils, and macrophages.
Alternative names	Interleukin 3, IL-3, IL3, colony-stimulating factor, multi-CSF, mast cell growth factor, MULTI-CSF, MCGF
Cell type	T cell, Th2
Gene ID	3562